Vaginal gram

The microbiological diagnosis of bacterial vaginosis is usually made using Nugent's criteria, a useful but rather laborious scoring system based on counting bacterial cell types on Gram stained slides of vaginal smears. Ison and Hay have simplified the score system to three categories and added a fourth category for microflora with a predominance of the Streptococcus cell type. Because in the Nugent system several cell types are not taken into account for a final score, we carried out a detailed assessment of the composition of the vaginal microflora in relation to standard Gram stain in order the improve the diagnostic value of the Gram stain.

Bacterial vaginosis BV is a highly prevalent condition associated with adverse health outcomes. Gram stain analysis of vaginal fluid is the standard for confirming the diagnosis of BV, wherein abundances of key bacterial morphotypes are assessed. These Lactobacillus, GardnerellaBacteroidesand Mobiluncus morphotypes were originally linked to particular bacterial species through cultivation studies, but no studies have systematically investigated associations between uncultivated bacteria detected by molecular methods and Gram stain findings.

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Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page. Aim —The status of vaginal lactobacillary flora, an indicator of possible genital infection and pregnancy complications, can be assessed on wet mount or Gram stained specimens.

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European Journal of Clinical Microbiology. The facultative and strictly anaerobic vaginal microbial flora was investigated in 40 women with non-specific vaginitis and in 40 control women seen in private gynaecological practice. The most common anaerobes were Veillonella parvula, Bacteroides bivius, Bacteroides assaccharolyticus, Bacteroides capillosus and Peptococcus asaccharolyticus.

Vaginal flora or vaginal microbiota are the microorganisms that colonize the vagina. The amount and type of bacteria present have significant implications for a woman's overall health. The primary colonizing bacteria of a healthy individual are of the genus Lactobacillus.

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Skip to search form Skip to main content. Diagnosis of bacterial vaginosis by direct gram stain of vaginal fluid. Spiegel and Rhonda Amsel and King K. SpiegelRhonda AmselKing K.

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NCBI Bookshelf. Boston: Butterworths; The normal vaginal discharge is white, nonhomogeneous, and viscous.


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